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COMPARATIVE ANALYSIS OF MORPHOPHYSIOLOGICAL TRAITS OF TRITICUM V. GERMINANTS AFTER EXPOSURE TO NANOPARTICLES
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A. Korotkova;O. Kvan;I. Gavrish;D. Kosyan;S. Lebedev
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1314-2704
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English
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19
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6.1
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We studied the effect of Cu and Ni nanoparticles on the plant Triticum vulgare. Observing the germination energy, we faced a decrease 16.7 times for Ni? and total suppression for Cu. These nanoparticles reduced the growth of seedlings and affected the placement of lateral roots, which grew like a ?goose-paw?.
The total sum of reactive oxygen species ended up with an increase in the roots after exposure to Cu? (up to 35.8% compared to the negative control). Expressed statistically significant (in comparison with the control (P <0.05)) accumulation of HO? when Cu was applied at a dose of 0.05 and 0.1 M (up to 8.5 and 13.6%). The cell viability and proliferation assays indicated that the toxic effects of nanoCu was maximum after 24 of exposure and that accounted 89% in comparison to control. The presence of Ni? in the medium resulted in a smoother formazan reduction after 24 hours of incubation (up to 56% relative to control). Thus, we obtained data indicating a decrease in viability of root cells under the action of nanometals, with the form of degradation of DNA molecules having either necrotic, unsystematic (in case of Cu) or internucleosomal apoptotic character (Ni). |
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conference
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19th International Multidisciplinary Scientific GeoConference SGEM 2019
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19th International Multidisciplinary Scientific GeoConference SGEM 2019, 30 June - 6 July, 2019
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Proceedings Paper
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STEF92 Technology
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International Multidisciplinary Scientific GeoConference-SGEM
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Bulgarian Acad Sci; Acad Sci Czech Republ; Latvian Acad Sci; Polish Acad Sci; Russian Acad Sci; Serbian Acad Sci & Arts; Slovak Acad Sci; Natl Acad Sci Ukraine; Natl Acad Sci Armenia; Sci Council Japan; World Acad Sci; European Acad Sci, Arts & Letters; Ac
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93-100
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30 June - 6 July, 2019
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website
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cdrom
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6362
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Triticum vulgare; nanoparticle; cell viability; DNA damage.
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