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Title: PREPARATION OF DRUG-LOADED PLGA NANOPARTICLES BY SPRAY-DRYING USING A BUCHI SPRAY DRYER B-90
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PREPARATION OF DRUG-LOADED PLGA NANOPARTICLES BY SPRAY-DRYING USING A BUCHI SPRAY DRYER B-90
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1314-2704
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English
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19
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6.1
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Previously we have developed the PLGA nanoparticles loaded with doxorubicin (PLGA-DOX NP) that demonstrated a high anti-tumor effect against the rat glioblastoma in the preclinical studies and is now being evaluated in the clinical phase II study. This formulation was obtained by a generally accepted homogenization ? solvent evaporation technique followed by freeze-drying. The purpose of the present study was to explore the feasibility to simplify the process of the nanoparticle preparation by replacing a freeze drying step with spray drying using a BUCHI Nano Spray Dryer B-90. The NP were prepared using the low molecular weight PLGA (Resomer® RG 502H) as a core polymer. The drug-to-polymer ratio was maintained at 1:10 w/w. The new process optimization involved variation of the following parameters: velocity of the air flow; inlet temperature; concentration of solid ingredients, ratio of aqueous and organic phases (w1:o:w2) in the initial emulsion; concentration of polyvinyl alcohol (PVA) used as stabilizer. Evaluation of the significance of the process parameters carried out by the Taguchi method revealed that the formulation factors had a greater impact on the nanoparticle parameters than technological ones. This result points to a comparatively easy configuration of the spray drying process for the PLGA nanoparticle-based formulations. Importantly, the characteristics of the nanoparticles were not affected by the drying gas at the temperatures required for efficient drying (80°C). The physicochemical parameters of the doxorubicin-loaded PLGA nanoparticles obtained by the optimized spray drying process (average size 230 ± 16 nm, PDI 0.213 ± 0.090, encapsulation efficiency of 85%) were similar to the parameters of the nanoparticles obtained by a conventional homogenization ? solvent evaporation - freeze-drying technique.
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conference
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19th International Multidisciplinary Scientific GeoConference SGEM 2019
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19th International Multidisciplinary Scientific GeoConference SGEM 2019, 30 June - 6 July, 2019
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Proceedings Paper
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STEF92 Technology
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International Multidisciplinary Scientific GeoConference-SGEM
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Bulgarian Acad Sci; Acad Sci Czech Republ; Latvian Acad Sci; Polish Acad Sci; Russian Acad Sci; Serbian Acad Sci & Arts; Slovak Acad Sci; Natl Acad Sci Ukraine; Natl Acad Sci Armenia; Sci Council Japan; World Acad Sci; European Acad Sci, Arts & Letters; Ac
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355-364
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30 June - 6 July, 2019
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website
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cdrom
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6397
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nanoparticles; spray-drying; PLGA; doxorubicin
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