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Title: IMMOBILIZATION OF GLUCOSE OXIDASE ON MAGNETIC MESOPOROUS OXIDES
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IMMOBILIZATION OF GLUCOSE OXIDASE ON MAGNETIC MESOPOROUS OXIDES
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1314-2704
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English
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21
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7.2
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• Prof. DSc. Oleksandr Trofymchuk, UKRAINE
• Prof. Dr. hab. oec. Baiba Rivza, LATVIA |
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The immobilization of glucose oxidase (GOx, enzyme of oxidoreductases class) was carried out on non-magnetic (SiO2, Al2O3) and magnetic mesoporous oxides (Fe3O4-SiO2, Fe3O4-Al2O3). Magnetic supports were synthesized by in-situ crystallization of magnetite nanoparticles in mesopores. The surface of the support was preliminarily modified with amino groups and the glutaraldehyde was used as a linker for covalent binding of the enzyme. The supports and biocatalysts were fully characterized using a combination of physicochemical methods (BET, TEM, DRIFT) and tested in D-glucose oxidation to D-gluconic acid. Magnetic support based biocatalysts demonstrate high catalytic activity in D-glucose oxidation at temperature of 30-50?C and pH 5-7.5 with the best activities of 95% and 91% for magnetic silica and alumina, respectively. A comparison of magnetic and non-magnetic alumina and silica shows a significant enhancement of the relative catalytic activity for magnetic supports.
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conference
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21st International Multidisciplinary Scientific GeoConference SGEM 2021
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21st International Multidisciplinary Scientific GeoConference SGEM 2021, 16 - 22 August, 2021
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Proceedings Paper
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STEF92 Technology
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SGEM International Multidisciplinary Scientific GeoConference
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SWS Scholarly Society; Acad Sci Czech Republ; Latvian Acad Sci; Polish Acad Sci; Serbian Acad Sci & Arts; Natl Acad Sci Ukraine; Natl Acad Sci Armenia; Sci Council Japan; European Acad Sci, Arts & Letters; Acad Fine Arts Zagreb Croatia; Croatian Acad Sci
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163-170
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16 - 22 August, 2021
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website
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cdrom
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8265
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magnetite nanoparticles; magnetic and non-magnetic mesoporous oxides; immobilization of glucose oxidase; D-glucose oxidation
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