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Title: APPLICATION OF CULTURE SUPERNATANTS CONTAINING SURFACTANTS TO ENHANCE OIL RECOVERY
APPLICATION OF CULTURE SUPERNATANTS CONTAINING SURFACTANTS TO ENHANCE OIL RECOVERY

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Liliya Biktasheva; Nikita Ezhkin; Zlata Osmorskaya; Alexander Gordeev; Polina Kuryntseva
10.5593/sgem2024/1.1/s06.67
10.5593/sgem2024/1.1
1314-2704
978-619-7603-67-5
English
24
1.1
•    Prof. DSc. Oleksandr Trofymchuk, UKRAINE 
•    Prof. Dr. hab. oec. Baiba Rivza, LATVIA
6.Oil and Gas Exploration
Currently, a big problem is increasing the production of residual oil, for which so-called tertiary recovery methods are used. One common tertiary recovery method is the use of surfactants. The use of biological surfactants instead of synthetic ones can solve the problem of increasing oil production and reducing the toxic impact on the environment. To obtain biosurfactants, two strains of Bacillus mojavensis and Bacillus amyloliquefaciens were used. These strains are characterized by the ability to produce biosurfactants. Biosurfactants produced by these strains belonged to the group of lipopeptides, represented mainly by surfactin molecules. The qPCR method established that in strains B. mojavensis and B. amyloliquefaciens the number of srfAA and ituC genes responsible for the production of surfactin was 2.8x104 and 1.9x103 gene copies ml-1, and iturin for 4.6x102 gene copies ml-1 in strain B amyloliquefaciens. To evaluate the effectiveness of MEOR, culture supernatant containing biosurfactants was tested on sand cores simulating oil reservoir. The surface tension of the culture supernatants of the B. mojavensis and B. amyloliquefaciens strains was 29.79 ± 0.039 and 31.18 ± 0.065 mN m-1. When culture supernatants were used, additional oil recovery was 11.1 and 11.8% for B. mojavensis and B. amyloliquefaciens, respectively.
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This work was supported by the Ministry of Science and Higher Education of the Russian Federation under agreement No. 075-15-2022-299 within the framework of the development program for a world-class research center “Efficient development of the global liquid hydrocarbon reserves”
conference
https://doi.org/10.5593/sgem2024/1.1/s06.67
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Proceedings of 24th International Multidisciplinary Scientific GeoConference SGEM 2024
24th International Multidisciplinary Scientific GeoConference SGEM 2024, 1 - 7 July, 2024
Proceedings Paper
STEF92 Technology
International Multidisciplinary Scientific GeoConference Surveying Geology and Mining Ecology Management, SGEM
SWS Scholarly Society; Acad Sci Czech Republ; Latvian Acad Sci; Polish Acad Sci; Russian Acad Sci; Serbian Acad Sci and Arts; Natl Acad Sci Ukraine; Natl Acad Sci Armenia; Sci Council Japan; European Acad Sci, Arts and Letters; Acad Fine Arts Zagreb Croatia; Croatian Acad Sci and Arts; Acad Sci Moldova; Montenegrin Acad Sci and Arts; Georgian Acad Sci; Acad Fine Arts and Design Bratislava; Russian Acad Arts; Turkish Acad Sci.
541-548
1 - 7 July, 2024
website
9896
Biosurfactants, microbial enhanced oil recovery, MEOR

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