Peer-reviewed articles 17,970 +



Title: ASSESSMENT OF THE BIODEGRADABILITY OF CATIONIC FLOCCULANTS BY MICROORGANISMS ISOLATED FROM SEWAGE SLUDGE OBTAINED ON WASTEWATER TREATMENT PLANTS

ASSESSMENT OF THE BIODEGRADABILITY OF CATIONIC FLOCCULANTS BY MICROORGANISMS ISOLATED FROM SEWAGE SLUDGE OBTAINED ON WASTEWATER TREATMENT PLANTS
Gulnaz Galieva; Polina Kuryntseva; Polina Galitskaya; Svetlana Selivanovskaya
10.5593/sgem2024/5.1
1314-2704
English
24
5.1
•    Prof. DSc. Oleksandr Trofymchuk, UKRAINE 
•    Prof. Dr. hab. oec. Baiba Rivza, LATVIA
Annually, a city with 500,000 inhabitants generates approximately 115.5 million cubic meters of wastewater. The treatment of this wastewater is efficiently managed using activated sludge, which comprises a diverse community of protozoa, infusoria, lower crustaceans, algae, and microorganisms. These organisms facilitate the effective treatment of large volumes of wastewater in treatment plants. However, the activated sludge proliferates by consuming organic materials in the wastewater, resulting in the accumulation of substantial volumes of sewage sludge in these plants. To manage this sludge, it is pre-dewatered using flocculants.
While flocculants are effective for dewatering, the presence of polyacrylamides poses a challenge due to their resistance to degradation and potential environmental toxicity. Therefore, before the biological treatment of sewage sludge, it is essential to purify it from flocculants. This study aimed to isolate bacteria capable of degrading cationic flocculants from the activated sludge of municipal wastewater treatment plants, screen their growth rates, identify the most promising microorganism species, and evaluate their ability to degrade cationic flocculants.
Two bacterial isolates were obtained, capable of growing on a medium where the sole carbon source was Greenlife K-35 and Mainfloc 5858 brand flocculants. The most effective isolate was identified based on growth curves. DNA sequencing of the 16S rRNA region by the Sanger method identified this strain as Pseudomonas nitroreducens. In the presence of this strain, the degradation rates of Mainfloc 5858 and Greenlife K-35 were 61.5% and 59.6% at 28 days, respectively, as evaluated using the OxiTop system.
[1] Liang Y., Wang R., Sun W., Sun Y., Advances in Chemical Conditioning of Residual Activated Sludge in China,Water 2023, Vol. 15, Page 345, 2023, vol. 15, pp. 345.
[2] Feng X., et al., Preparation of a hydrophobically associated cationic polyacrylamide and its regulation of the sludge dewatering performance,Water Science and Technology, 2020, vol. 82, pp. 1350–1369.
[3] Doble M., Kumar A., Degradation of Polymers,Biotreatment of Industrial Effluents, 2005, pp. 101–110.
[4] Guezennec A. G., et al., Transfer and degradation of polyacrylamide-based flocculants in hydrosystems: A review,Environmental Science and Pollution Research, 2015, vol. 22, pp. 6390–6406.
[5] Ma F., Wei L., Wang L., Chang C. C., Isolation and identification of the sulphate-reducing bacteria strain H1 and its function for hydrolysed polyacrylamide degradation,International Journal of Biotechnology, 2008, vol. 10, pp. 55–63.
[6] Brown D. M., Hughes C. B., Spence M., Bonte M., Whale G., Assessing the suitability of a manometric test system for determining the biodegradability of volatile hydrocarbons,Chemosphere, 2018, vol. 195, pp. 381–389.
[7] Crossley B. M., et al., Guidelines for Sanger sequencing and molecular assay monitoring,Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, 2020, vol. 32, pp. 767.
[8] Janda J. M., Abbott S. L., 16S rRNA Gene Sequencing for Bacterial Identification in the Diagnostic Laboratory: Pluses, Perils, and Pitfalls,Journal of Clinical Microbiology, 2007, vol. 45, pp. 2761.
[9] Carraro N., Sentchilo V., Polak L., Bertelli C., van der Meer J. R., Insights into Mobile Genetic Elements of the Biocide-Degrading Bacterium Pseudomonas nitroreducens HBP-1,Genes 2020, Vol. 11, Page 930, 2020, vol. 11, pp. 930.
[10] Lawrence R. N., et al., The Pseudomonas aeruginosa quorum-sensing signal molecule, N-(3-oxododecanoyl)-L-homoserine lactone, inhibits porcine arterial smooth muscle contraction,British Journal of Pharmacology, 1999, vol. 128, pp. 845.
[11] Trinh C. S., et al., Evaluation of the plant growth-promoting activity of Pseudomonas nitroreducens in Arabidopsis thaliana and Lactuca sativa,Plant cell reports, 2018, vol. 37, pp. 873–885.
[12] Bao M., Chen Q., Li Y., Jiang G., Biodegradation of partially hydrolyzed polyacrylamide by bacteria isolated from production water after polymer flooding in an oil field,Journal of hazardous materials, 2010, vol. 184, pp. 105–110.
The work is carried out in accordance with the Strategic Academic Leadership Program "Priority 2030" of the Kazan Federal University of the Government of the Russian Federation.
conference
Proceedings of 24th International Multidisciplinary Scientific GeoConference SGEM 2024
24th International Multidisciplinary Scientific GeoConference SGEM 2024, 1 - 7 July, 2024
Proceedings Paper
STEF92 Technology
International Multidisciplinary Scientific GeoConference Surveying Geology and Mining Ecology Management, SGEM
SWS Scholarly Society; Acad Sci Czech Republ; Latvian Acad Sci; Polish Acad Sci; Russian Acad Sci; Serbian Acad Sci and Arts; Natl Acad Sci Ukraine; Natl Acad Sci Armenia; Sci Council Japan; European Acad Sci, Arts and Letters; Acad Fine Arts Zagreb Croatia; Croatian Acad Sci and Arts; Acad Sci Moldova; Montenegrin Acad Sci and Arts; Georgian Acad Sci; Acad Fine Arts and Design Bratislava; Russian Acad Arts; Turkish Acad Sci.
17-24
1 - 7 July, 2024
website
9953
sewage sludge, biodegradability, cationic flocculant, OxiTop® measuring systems

25th SGEM International Conference on Earth & Planetary Sciences


International GeoConference SGEM2025
27 June - 6 July, 2025 / Albena, Bulgaria

Read More
   

SGEM Vienna GREEN "Green Science for Green Life"


Extended Scientific Sessions SGEM Vienna GREEN
3 -6 December, 2025 / Vienna, Austria

Read More
   

A scientific platform for Art-Inspired Scientists!


The Magical World Where Science meets Art
Vienna, Austria

Read More