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Oxidoreductase enzymes play a key role in modern biotechnology due to their high specificity and ability to catalyze redox processes under mild conditions. They are used in environmental monitoring, biosensor development, and fine organic synthesis. However, their practical use in their native form is limited by their low stability, sensitivity to environmental changes, and the inability to re-extract from the reaction mixture. Immobilizing enzymes on various carriers can solve these problems. Magnetic nanoparticl...

A new biocatalyst based on glucose oxidase immobilized on Fe3O4 magnetite nanoparticles modified with chitosan and sodium tripolyphosphate was synthesized. Magnetite nanoparticles were obtained by mixing solutions of FeCl2 and FeCl3 with ammonia while heating to a temperature of 65 ?C. To stabilize the nanoparticles and ensure the presence of amino groups on their surface, chitosan and sodium tripolyphosphate were sequentially deposited on magnetite. Immobilization of glucose oxidase on the support was carried out...

The immobilization of glucose oxidase (GOx, enzyme of oxidoreductases class) was carried out on non-magnetic (SiO2, Al2O3) and magnetic mesoporous oxides (Fe3O4-SiO2, Fe3O4-Al2O3). Magnetic supports were synthesized by in-situ crystallization of magnetite nanoparticles in mesopores. The surface of the support was preliminarily modified with amino groups and the glutaraldehyde was used as a linker for covalent binding of the enzyme. The supports and biocatalysts were fully characterized using a combination of physi...

Porous supports including oxides and polymers have been employed for enzyme immobilization for many years. In recent times, the research focus has been placed on hierarchical structures, dependencies on pore sizes, and the use of magnetic porous oxides for magnetic recovery. The presence of pores or cavities where enzymes could locate to create an optimal degree of crowding achieved in cells is not a prerequisite, but it could significantly enhance the catalytic activity of biocatalysts. For the last five years, t...