Browse

The study presents the comparative analysis between pressure and carbon dioxide charts obtained after measuring a series of fermentation processes. Doughs used for measurements were obtained from different quality/extraction flours, type 650 and whole wheat (1850). Gathered data was used to verify a mathematical model for determination of carbon dioxide production during the fermentation process, using pressure variation in a sealed container (reactor). For the testing it was used an experimental stand composed of...

The study presents the comparative analysis between pressure and carbon dioxide charts obtained after measuring a series of fermentation processes. Doughs used for measurements were obtained from different quality/extraction flours, type 650 and whole wheat (1850). Gathered data was used to verify a mathematical model for determination of carbon dioxide production during the fermentation process, using pressure variation in a sealed container (reactor). For the testing it was used an experimental stand composed of...

The aim of the study was to compare the functional and technological properties of protein concentrates (PC), protein-calcium composites (PCC) and protein concentrates hydrolyzed by endoprotease and exopeptidase (HPC) with a degree of proteolysis of 3.89-4.16%. All kinds of concentrates were obtained from white and brown rice. It was found that the functional properties are higher in BC obtained from white rice than in brown rice rice. Thus, the foaming capacity is 5-6 times higher, but the stability of foam in br...

The aim of the study was to compare the functional and technological properties of protein concentrates (PC), protein-calcium composites (PCC) and protein concentrates hydrolyzed by endoprotease and exopeptidase (HPC) with a degree of proteolysis of 3.89-4.16%. All kinds of concentrates were obtained from white and brown rice. It was found that the functional properties are higher in BC obtained from white rice than in brown rice rice. Thus, the foaming capacity is 5-6 times higher, but the stability of foam in br...

Waste products of plant origin are valuable secondary raw materials in the process of agricultural wastes utilization into products with high added value. The application of secondary raw materials is complicated due to the high fibrecontent. The aim of this study was to identify strains of microorganisms capable to produce a wide range of exocellulolases in order to reduce the crude fibrein mild conditions without applying expensive commercial cellulases. The following strains were chosen for this experiment: Tri...

Waste products of plant origin are valuable secondary raw materials in the process of agricultural wastes utilization into products with high added value. The application of secondary raw materials is complicated due to the high fibrecontent. The aim of this study was to identify strains of microorganisms capable to produce a wide range of exocellulolases in order to reduce the crude fibrein mild conditions without applying expensive commercial cellulases. The following strains were chosen for this experiment: Tri...

The fast development in chromatography occurred in the middle of twentieth century when gas chromatography came to light. It was invented by James and Martin. Presently, gas chromatography coupled with universal or selective detectors including mass selective detector is an effective, powerful and versatile analytical tool for special cases. It can be applied in numerous fields for example in drugs and pharmaceuticals, clinical chemistry, foods, environmental analysis or petroleum industry. Gas chromatography can ...

The fast development in chromatography occurred in the middle of twentieth century when gas chromatography came to light. It was invented by James and Martin. Presently, gas chromatography coupled with universal or selective detectors including mass selective detector is an effective, powerful and versatile analytical tool for special cases. It can be applied in numerous fields for example in drugs and pharmaceuticals, clinical chemistry, foods, environmental analysis or petroleum industry. Gas chromatography can ...

The study of halobacterial communities is a promising direction in applied and theoretical biotechnology. Researches, carried out in this field, open up new approaches in understanding the role of interspecies interactions between halobacterial cells in conditions of increased salinity. Viruses, as one of the major regulation factor, greatly affect the composition of communities. Every year the halovirus database is replenished with new representatives, but still much remains unknown about the life cycle of viruse...

The study of halobacterial communities is a promising direction in applied and theoretical biotechnology. Researches, carried out in this field, open up new approaches in understanding the role of interspecies interactions between halobacterial cells in conditions of increased salinity. Viruses, as one of the major regulation factor, greatly affect the composition of communities. Every year the halovirus database is replenished with new representatives, but still much remains unknown about the life cycle of viruse...

The stoichiometry of metal?protein complexes is pH dependent. Since mass spectrometric measurements at higher pH values are difficult in the presence of usual buffers, we extended this approach to pH in excess of 10 by adding ethanolamine to the electrospray solution being investigated. Therefore, a new method for probing the metal ion-peptide complexes at high pH utilizing electrospray ionization mass spectrometry (ESI-MS) has been advanced. Nevertheless, the high pH ESI-MS spectra of peptides are far too complex...

The stoichiometry of metal?protein complexes is pH dependent. Since mass spectrometric measurements at higher pH values are difficult in the presence of usual buffers, we extended this approach to pH in excess of 10 by adding ethanolamine to the electrospray solution being investigated. Therefore, a new method for probing the metal ion-peptide complexes at high pH utilizing electrospray ionization mass spectrometry (ESI-MS) has been advanced. Nevertheless, the high pH ESI-MS spectra of peptides are far too complex...

Alzheimer?s disease (AD) is associated with conformational changes of amyloid-? peptides, which result in peptide oligomerization and fibril formation and, finally, in the appearance of senile plaques and extensive neuronal loss. However, the factors regulating the process of plaque-associated beta-amyloid peptide (A?) aggregation are only partly understood. Therefore, several ?-amyloid peptide fragments have been synthesized by SPPS according to Boc/Bzl strategy. Binding of metal ions to ?-amyloid peptides was st...

Alzheimer?s disease (AD) is associated with conformational changes of amyloid-? peptides, which result in peptide oligomerization and fibril formation and, finally, in the appearance of senile plaques and extensive neuronal loss. However, the factors regulating the process of plaque-associated beta-amyloid peptide (A?) aggregation are only partly understood. Therefore, several ?-amyloid peptide fragments have been synthesized by SPPS according to Boc/Bzl strategy. Binding of metal ions to ?-amyloid peptides was st...

Molluscs are invertebrates that form one of the largest groups in the animal kingdom, with more than 100,000 known species. A wide range of molluscs including families of Pulmonata, Gastropoda, Planorbidae and Lymnaeidae are intermediate hosts of trematodes. The snail Lymnaea stagnalis belongs to the family Lymnaeidae under the order Basommatophora-class Pulmonata is known to be an intermediate host of several parasites such as Chaetogaster limnaei which belongs to the annelid family, Naididae, an ecologically div...

Molluscs are invertebrates that form one of the largest groups in the animal kingdom, with more than 100,000 known species. A wide range of molluscs including families of Pulmonata, Gastropoda, Planorbidae and Lymnaeidae are intermediate hosts of trematodes. The snail Lymnaea stagnalis belongs to the family Lymnaeidae under the order Basommatophora-class Pulmonata is known to be an intermediate host of several parasites such as Chaetogaster limnaei which belongs to the annelid family, Naididae, an ecologically div...

The magnetically recoverable biocatalyst was developed. The catalyst support is based on magnetite particles (MPs) composed of magnetite particles and coated with the amino terminated silica layer to facilitate further functionalization. This functionalization involves the attachment of the glutaraldehyde linker followed by the covalent attachment of horseradish peroxidase (HRP) via its amino groups. The catalyst was tested in the reaction of 2,3,6-trimethylphenol (TMP) oxidation to 2,3,5-trimethylhydrochinone (TM...

The magnetically recoverable biocatalyst was developed. The catalyst support is based on magnetite particles (MPs) composed of magnetite particles and coated with the amino terminated silica layer to facilitate further functionalization. This functionalization involves the attachment of the glutaraldehyde linker followed by the covalent attachment of horseradish peroxidase (HRP) via its amino groups. The catalyst was tested in the reaction of 2,3,6-trimethylphenol (TMP) oxidation to 2,3,5-trimethylhydrochinone (TM...

Food habits are specific to each nationality. One common point is represented by the dairy products due to their specific properties. Starting from the raw material used (milk) which could ensure all the nutrients needed for supporting life, the dairy conserves most of the milk components but with a high preservation degree and digestibility. The present study focuses on establishing the nutritional and toxicological characteristics of some local traditionally made cheese. The motivation of the samples taken is de...

Food habits are specific to each nationality. One common point is represented by the dairy products due to their specific properties. Starting from the raw material used (milk) which could ensure all the nutrients needed for supporting life, the dairy conserves most of the milk components but with a high preservation degree and digestibility. The present study focuses on establishing the nutritional and toxicological characteristics of some local traditionally made cheese. The motivation of the samples taken is de...

The work has been carried out during 2016 ? 2017 period, in a vineyard located in Valea Calugareasca viticultural center, aiming to developing a "smart" and "automated" irrigation system for vine, which allows the use of both rain and irrigation water with maximum efficiency, when the pluviometric water can not satisfy the water needs of the grapevine. The researches were carried out within a demonstration polygon with the T?m?ioasa rom?neasc? variety and aimed to determine the influence of two irrigation systems ...

The work has been carried out during 2016 ? 2017 period, in a vineyard located in Valea Calugareasca viticultural center, aiming to developing a "smart" and "automated" irrigation system for vine, which allows the use of both rain and irrigation water with maximum efficiency, when the pluviometric water can not satisfy the water needs of the grapevine. The researches were carried out within a demonstration polygon with the T?m?ioasa rom?neasc? variety and aimed to determine the influence of two irrigation systems ...

Salvia species are parts of the daily routine of many communities in different regions of the world (Southern and Central Europe, North America, Middle East, etc.). Many research studies show a strong correlation between the concentration of some biologically active substances such as terpenoids, alkaloids, polyphenols and their antimicrobial action. The presence of these chemical compounds resulting from the secondary metabolism of the plant has determined that sage is of great interest to the food industry and i...

Salvia species are parts of the daily routine of many communities in different regions of the world (Southern and Central Europe, North America, Middle East, etc.). Many research studies show a strong correlation between the concentration of some biologically active substances such as terpenoids, alkaloids, polyphenols and their antimicrobial action. The presence of these chemical compounds resulting from the secondary metabolism of the plant has determined that sage is of great interest to the food industry and i...

Environmental pollution represents one of the serious problems of our society. During anthropogenic activities (mining and ore processing, metal processing industry) toxic compounds and heavy metals are introduced into our environment. Their accumulation in ecosystems creates a huge environmental load and it threats environment and human health. Another emerging environmental threat is a genetic pollution. Genetic pollution is an uncontrolled gene (usually antibiotic resistance gene) flow into populations of non-t...

Environmental pollution represents one of the serious problems of our society. During anthropogenic activities (mining and ore processing, metal processing industry) toxic compounds and heavy metals are introduced into our environment. Their accumulation in ecosystems creates a huge environmental load and it threats environment and human health. Another emerging environmental threat is a genetic pollution. Genetic pollution is an uncontrolled gene (usually antibiotic resistance gene) flow into populations of non-t...

Two technological factors were taken into study: loadings: 20, 28 and 36 eyes/vine, with the attribution of a cutting load of 8-10 eyes per vine and two eyes taps and the maceration-fermentation duration of the wine from marc, respectively 8 and 16 days. Feteasca neagra and Cabernet Sauvignon varieties, part of the basic assortment for the production of quality red wines, typicaly for Dealu Mare vineyard, were used. The polyphenol accumulation rate over the maceration-fermentation time registered values between 58...

Two technological factors were taken into study: loadings: 20, 28 and 36 eyes/vine, with the attribution of a cutting load of 8-10 eyes per vine and two eyes taps and the maceration-fermentation duration of the wine from marc, respectively 8 and 16 days. Feteasca neagra and Cabernet Sauvignon varieties, part of the basic assortment for the production of quality red wines, typicaly for Dealu Mare vineyard, were used. The polyphenol accumulation rate over the maceration-fermentation time registered values between 58...

Winter pumpkin represents a seasonal crop of health and food interest due to its nutritional composition. The aim of the present study was to comparatively evaluate the content of the main antioxidant compounds, carotenoids and phenolics, from pumpkin (Cucurbita maxima) pulp as related to different pretreatment conditions. Samples of pumpkin pulp were subjected to three pre-extraction methods, as follow: freeze-drying, oven-drying at 60oC and freezing at -70oC. Conventional extraction of carotenoids and polyphenol...

Winter pumpkin represents a seasonal crop of health and food interest due to its nutritional composition. The aim of the present study was to comparatively evaluate the content of the main antioxidant compounds, carotenoids and phenolics, from pumpkin (Cucurbita maxima) pulp as related to different pretreatment conditions. Samples of pumpkin pulp were subjected to three pre-extraction methods, as follow: freeze-drying, oven-drying at 60oC and freezing at -70oC. Conventional extraction of carotenoids and polyphenol...

The use of various ingredients in the formulation of yogurt, in order to diversify a dairy product particularly appreciated by consumers, is a constant concern for industry specialists. The paper proposes the use pumpkin seeds in the recipe of a classic yogurt, in order to improve its physico-chemical, textural and sensory characteristics. Pumpkin seeds, like other oilseeds, are in the attention of specialists because they are a very good source of proteins and dietary fibers. For the consumer, the increase in the...

The use of various ingredients in the formulation of yogurt, in order to diversify a dairy product particularly appreciated by consumers, is a constant concern for industry specialists. The paper proposes the use pumpkin seeds in the recipe of a classic yogurt, in order to improve its physico-chemical, textural and sensory characteristics. Pumpkin seeds, like other oilseeds, are in the attention of specialists because they are a very good source of proteins and dietary fibers. For the consumer, the increase in the...

The researches were carried out in two vineyards grown with the Feteasca neagra and Cabernet Sauvignon varieties, with two experimental factors: the soil maintenance system (black furrow, total mulching with straws, partial mulching with marc compost, permanent herbage with grasses spontaneous flora) and the system for the control of pests and diseases (ecologically and conventional). The experimental results obtained showed that under the ecoclimatic conditions specific to 2017 year, characterized by a high helio...

The researches were carried out in two vineyards grown with the Feteasca neagra and Cabernet Sauvignon varieties, with two experimental factors: the soil maintenance system (black furrow, total mulching with straws, partial mulching with marc compost, permanent herbage with grasses spontaneous flora) and the system for the control of pests and diseases (ecologically and conventional). The experimental results obtained showed that under the ecoclimatic conditions specific to 2017 year, characterized by a high helio...

Kraft and sulphite wood pulps are good substrates for enzymatic sugars production. Drying is a necessary step in commercial pulp, cardboard and paper products manufacturing. During drying significant changes occur in the structure of cellulose fibers. It is associated with compression and hornification processes, which have a significant effect on fiber properties. The aim of the work was to evaluate the effect of wood pulp drying on its enzymatic hydrolysability. Softwood and hardwood kraft pulps, softwood sulphi...

Kraft and sulphite wood pulps are good substrates for enzymatic sugars production. Drying is a necessary step in commercial pulp, cardboard and paper products manufacturing. During drying significant changes occur in the structure of cellulose fibers. It is associated with compression and hornification processes, which have a significant effect on fiber properties. The aim of the work was to evaluate the effect of wood pulp drying on its enzymatic hydrolysability. Softwood and hardwood kraft pulps, softwood sulphi...

The field of biotechnology provides manageable production of useful products for various spheres of human activity, based on the use of various biological resources and systems, as well as extracellular substances and cell components. The development and transformation of biotechnology is due to active research and continuous application of new technologies. Numerous studies of recent decades have convincingly shown that the actual task is to create an information database that will contain information of prebioti...

The field of biotechnology provides manageable production of useful products for various spheres of human activity, based on the use of various biological resources and systems, as well as extracellular substances and cell components. The development and transformation of biotechnology is due to active research and continuous application of new technologies. Numerous studies of recent decades have convincingly shown that the actual task is to create an information database that will contain information of prebioti...

The paper resumes the previously published series of studies on the efficacy of a dry fractioning operation in centrifugal field of forces on the complex lipid mixture of pork lard, by multivariate analysis in general and the principal component analysis (PCA). Particularly, the correlation between the operating parameters (time-temperature-centrifugal rotor speed) and the physic-chemical parameters was determinate. Changes induced to the lipid mixture by repeated fractional crystallization, to separate (olein / s...

The paper resumes the previously published series of studies on the efficacy of a dry fractioning operation in centrifugal field of forces on the complex lipid mixture of pork lard, by multivariate analysis in general and the principal component analysis (PCA). Particularly, the correlation between the operating parameters (time-temperature-centrifugal rotor speed) and the physic-chemical parameters was determinate. Changes induced to the lipid mixture by repeated fractional crystallization, to separate (olein / s...

Zinc oxide nanoparticles (ZnO) are a promising material for a variety of applications due to their unique characteristics and properties, including optical, chemical, semiconductor and piezoelectric. A lot of scientific papers are devoted to the antibacterial action of ZnO nanoparticles. As the main mechanism responsible for the antimicrobial action of ZnO in modern studies, photocatalytic generation of hydrogen peroxide H2O2 is indicated. In addition, in a number of studies it was found that the slowing of bacter...

Zinc oxide nanoparticles (ZnO) are a promising material for a variety of applications due to their unique characteristics and properties, including optical, chemical, semiconductor and piezoelectric. A lot of scientific papers are devoted to the antibacterial action of ZnO nanoparticles. As the main mechanism responsible for the antimicrobial action of ZnO in modern studies, photocatalytic generation of hydrogen peroxide H2O2 is indicated. In addition, in a number of studies it was found that the slowing of bacter...

Preservation at positive temperatures is limited in time. The common technique to reduce metabolism is by reducing the temperature below ambient, using refrigeration or cryogenics. Therefore, these methods are used to preserve biological matter (cells, tissues, organs, vegetables, fruits etc.). Preservation at negative temperatures is possible with limitations in time and imposes also other biotechnological challenges. Long term preservation below freezing temperatures is not successful yet for organs and tissues....

Preservation at positive temperatures is limited in time. The common technique to reduce metabolism is by reducing the temperature below ambient, using refrigeration or cryogenics. Therefore, these methods are used to preserve biological matter (cells, tissues, organs, vegetables, fruits etc.). Preservation at negative temperatures is possible with limitations in time and imposes also other biotechnological challenges. Long term preservation below freezing temperatures is not successful yet for organs and tissues....

The pancreas of cattle is one of the most important by-products produced in the meat processing industry. Meat processing plants of medium capacity per year produce at least 40 tons of pancreas of cattle. The pancreas is a valuable raw material for the biotechnology, because it has high contents of hydrolytic enzymes. Among them, the most important enzymes are ribonuclease, proteases, amylase and lipase. The most perspective is the implementation of low-waste technology, involving the isolation of the maximum numb...

The pancreas of cattle is one of the most important by-products produced in the meat processing industry. Meat processing plants of medium capacity per year produce at least 40 tons of pancreas of cattle. The pancreas is a valuable raw material for the biotechnology, because it has high contents of hydrolytic enzymes. Among them, the most important enzymes are ribonuclease, proteases, amylase and lipase. The most perspective is the implementation of low-waste technology, involving the isolation of the maximum numb...

In the last years, due to its benefits, the syrup has gained a valuable place on the market, can be extracted from many flowers and medicinal plants, and from all kinds of fruits that can be squeezed out. Many studies have highlighted the beneficial therapeutic effect of fruit and herbal syrups as a cure for various diseases. Due to their curative properties, being good detoxifiers, general tonic and immunostimulating, the syrups have a major importance in modern food, having a dual functionality, namely that of f...

In the last years, due to its benefits, the syrup has gained a valuable place on the market, can be extracted from many flowers and medicinal plants, and from all kinds of fruits that can be squeezed out. Many studies have highlighted the beneficial therapeutic effect of fruit and herbal syrups as a cure for various diseases. Due to their curative properties, being good detoxifiers, general tonic and immunostimulating, the syrups have a major importance in modern food, having a dual functionality, namely that of f...

The aim of study was development of method of processing native starch by low-temperature biocatalysis without preliminary gelatinization, provides the production of new types of starch products. Was made studies of glucoamylase effect on native starch in water environment with temperature lower than sub-gelatinization temperature point. The objects of the research were samples of various types of starch (wheat, potato, maize native, amylopectin) and products of biocatalysis: modified starch and liquid fraction of...

The aim of study was development of method of processing native starch by low-temperature biocatalysis without preliminary gelatinization, provides the production of new types of starch products. Was made studies of glucoamylase effect on native starch in water environment with temperature lower than sub-gelatinization temperature point. The objects of the research were samples of various types of starch (wheat, potato, maize native, amylopectin) and products of biocatalysis: modified starch and liquid fraction of...