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The present work is an attempt to create a biosurfactant producing consortia on the bases of initial rhizospheric community of lettuce plant (Lactuca sativa). To obtain consortia, 47 strains from the rhizosphere were isolated and checked upon their ability to produce biosurfactants. The ability of the isolates to produce biosurfactants was analyzed on the basis of their ability to emulsify crude oil (E24 index). The isolates with the highest E24 were Bacillus. oryzaecorticis (80%), B. simplex (65%), Paenibacillus ...