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Annually, a city with 500,000 inhabitants generates approximately 115.5 million cubic meters of wastewater. The treatment of this wastewater is efficiently managed using activated sludge, which comprises a diverse community of protozoa, infusoria, lower crustaceans, algae, and microorganisms. These organisms facilitate the effective treatment of large volumes of wastewater in treatment plants. However, the activated sludge proliferates by consuming organic materials in the wastewater, resulting in the accumulation...

Biochar is a highly porous pyrolysis product with a high specific surface area and adsorption properties, capable of adsorbing various compounds and formations, including ARG-containing MGEs. To assess the ability of biochar to adsorb MGE, a model experiment was carried out: in sterill flasks, the microbial community isolated from composts was cultivated on LB-medium, oxytetracycline and a mixture of heavy metals were added as factors potentially stimulating the production of MGE. Neither heavy metals nor oxytetra...

Soil contamination by polycyclic aromatic hydrocarbons (PAHs) is a widespread concern due to the high potential for human exposure through inhalation, ingestion, and dermal contact. In this study, we compared the efficiency of three different methods for PAHs extraction from model samples, including ultrasonic (US), mechanical shaking and purification with commercial QuEChERS kit (Shq), and ultrasonic extraction coupled with QuEChERS (USq) by gas chromatography coupled to a triple quadrupole tandem mass-spectromet...

Surfactants play an important role in enhanced oil recovery by creating an oil-water emulsion that will be easier to recover from the reservoir. It is known that sulfatereducing bacteria (SRB) can use surfactants as a carbon source, which can increase the biological accumulation of sulfides and, accordingly, the corrosion of oil production equipment. Thus, all new surfactants used in oil production need to be pre-tested for SRB. The aim of this study was to evaluate the effect of four new groups of synthesized sur...

The use of microalgae as carbon sequestrators requires optimization of the cultivation process. Growth curves for three types of microalgae were plotted. The growth was carried out at different temperatures 30 and 35 ? and with initially different concentrations of inoculum 30 and 3% in order to assess the possibility of growing crops at the lowest cost and with a high yield of a useful product. We used 3 strains of microalgae - potential sequestrators. The introduction of the inoculum at a dose of 30% led to an a...

Pollution of aquatic ecosystems with antibiotic-resistant genes originating from human medicine and veterinary is an urgent problem due to the potential threaten to public health. Antibiotic-resistant genes enter surface waters and wastewater through vertical and horizontal water runoff. At the same time, heavy metals and biogenic substances often presented in aqueous ecosystems often exacerbate the problem since the drive the horizontal transfer of antibiotic-resistance genes. To solve the problem of purification...

The anthropogenic influence on petroleum reservoirs during oil extraction leads to the emergence of new electron acceptors and donors and exogenous microorganisms. Microbial biodegradation of hydrocarbons in petroleum reservoirs leads to a change of its composition, resulting in a decrease in its cost. The identification of microorganisms capable of in-situ degradation of hydrocarbons under reservoir conditions is critical to understand the role of microbes in hydrocarbon conversion. To analyze microbial biodegrad...

Public gardens and park zones are an important and widely visited component of urban spaces worldwide, however they can be polluted and even dangerous for humans and animals visiting them. The simplest way to screen the level of pollution of those green zones is to assess their ecological toxicity. In the present study, to assess the ecotoxicity of soil and ground samples obtained in 15 parks and public gardens in the city of Kazan (Russia) with 1 Mio citizens were investigated. Two elutriate assays with Ceriodaph...

The present work is an attempt to create a biosurfactant producing consortia on the bases of initial rhizospheric community of lettuce plant (Lactuca sativa). To obtain consortia, 47 strains from the rhizosphere were isolated and checked upon their ability to produce biosurfactants. The ability of the isolates to produce biosurfactants was analyzed on the basis of their ability to emulsify crude oil (E24 index). The isolates with the highest E24 were Bacillus. oryzaecorticis (80%), B. simplex (65%), Paenibacillus ...

The number of registered cases of COVID-19 is increasing in the world, and some countries are reporting a second wave of the pandemic. Accurate and real time information about epidemiological situation is therefore urgently needed for managing decisions in the countries, regions and municipalities which are affected. Massive testing of viral presence in people’s saliva, a smear from the nose, nasopharynx and / or oropharynx, bronchial lavage water obtained by fibrobronchoscopy (bronchoalveolar lavage), as well as ...

The problem of the antibiotic resistance of bacteria inhabiting the guts of poultry and livestock and furthermore manures and fertilizers prepared from manures is recognized as one of the most serious problems in agriculture. Composting is described as an effective way to reduce the abundance of antibiotic-resistant genes in the final product, however such a positive effect is not always guaranteed. The addition of highly porous components like biochar into composting mixtures is applied nowadays in order to impro...