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The biotechnological methods have wide application area in the remediation of heavy metals from the waste waters. This study presents the results of the biological-chemical activation of coal waste from the Cigel deposit (Slovakia) by selected species of microorganisms, as well as: Aspergillus niger, Asperg illus clavatus, Penicillium glabrum and Trichoderma viride . The quantitative maceral composition has been investigated. The complex maceral analysis shoved that microorganisms preferentially disrupt the struct...